{"id":114303,"date":"2024-10-15T06:01:48","date_gmt":"2024-10-14T23:01:48","guid":{"rendered":"https:\/\/hotvideos24.online\/?p=114303"},"modified":"2024-10-15T06:01:48","modified_gmt":"2024-10-14T23:01:48","slug":"key-protein-regulates-dna-enzymes-supporting-genome-stability","status":"publish","type":"post","link":"https:\/\/hotvideos24.online\/?p=114303","title":{"rendered":"Key Protein Regulates DNA Enzymes, Supporting Genome Stability"},"content":{"rendered":"<p> <script async src=\"https:\/\/pagead2.googlesyndication.com\/pagead\/js\/adsbygoogle.js?client=ca-pub-3711241968723425\"\r\n     crossorigin=\"anonymous\"><\/script>\r\n<ins class=\"adsbygoogle\"\r\n     style=\"display:block\"\r\n     data-ad-format=\"fluid\"\r\n     data-ad-layout-key=\"-fb+5w+4e-db+86\"\r\n     data-ad-client=\"ca-pub-3711241968723425\"\r\n     data-ad-slot=\"7910942971\"><\/ins>\r\n<script>\r\n     (adsbygoogle = window.adsbygoogle || []).push({});\r\n<\/script><br \/>\n<\/p>\n<div>\n<p><strong>Summary: <\/strong>Researchers have discovered that the protein USP50 regulates DNA replication by managing which enzymes\u2014nucleases or helicases\u2014cleave or unwind DNA strands during replication. This control is crucial for stable replication, especially when the process encounters issues that need restarting. When USP50 is absent, cells struggle to coordinate enzyme use, leading to replication errors and potential genetic instability.<\/p>\n<p>The findings provide new insights into genome maintenance and may help explain some hereditary conditions, such as early-onset aging and certain cancers. Understanding USP50\u2019s role opens doors to potential therapeutic strategies aimed at protecting DNA integrity.<\/p>\n<p><strong>Key Facts<\/strong><\/p>\n<ul class=\"wp-block-list\">\n<li>USP50 regulates enzyme selection during DNA replication, promoting stability.<\/li>\n<li>Without USP50, cells improperly deploy enzymes, leading to DNA replication defects.<\/li>\n<li>USP50\u2019s role is linked to understanding hereditary conditions and potential therapies.<\/li>\n<\/ul>\n<p><strong>Source: <\/strong>University of Birmingham<\/p>\n<p><strong>A protein that is involved in determining which enzymes cut or unwind DNA during the replication process has been identified in a new study.<\/strong><\/p>\n<p>In a new paper published in\u00a0<em>Nature Communications,<\/em>\u00a0an international team of researchers have found that the protein USP50 supports the DNA replication process by helping to decide the proper use of nucleases or helicases.<\/p>\n<figure class=\"wp-block-image size-full\"><picture fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-105420\"><source type=\"image\/webp\" srcset=\"https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience.jpg.webp 1200w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-300x200.jpg.webp 300w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-770x513.jpg.webp 770w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-1155x770.jpg.webp 1155w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-370x247.jpg.webp 370w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-293x195.jpg.webp 293w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-150x100.jpg.webp 150w\" sizes=\"(max-width: 1200px) 100vw, 1200px\"\/><img fetchpriority=\"high\" decoding=\"async\" width=\"1200\" height=\"800\" src=\"https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience.jpg\" alt=\"This shows DNA.\" srcset=\"https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience.jpg 1200w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-300x200.jpg 300w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-770x513.jpg 770w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-1155x770.jpg 1155w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-370x247.jpg 370w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-293x195.jpg 293w, https:\/\/neurosciencenews.com\/files\/2024\/10\/dna-enzyme-genetics-neuroscience-150x100.jpg 150w\" sizes=\"(max-width: 1200px) 100vw, 1200px\"\/> <\/picture><figcaption class=\"wp-element-caption\">The study also found that when USP50 is absent during replication activity, cells attempted to use various nucleases and helicases in a less coordinated fashion leading to replication defects in cells. Credit: Neuroscience News<\/figcaption><\/figure>\n<p>These enzymes are implemented during the DNA replication process to promote ongoing replication and where the copying machinery runs into problems and needs to restart.<\/p>\n<p>The team led by Professor Jo Morris from the University of Birmingham\u2019s Department of Cancer and Genomic Sciences have identified that USP50 determines which or the many helicases and nucleases are used during ongoing replication, fork restart, and the maintenance of telemores, the DNA-rich protein structures on the ends of chromosomes.<\/p>\n<p>The identification of USP50\u2019s role provides a new insight into the DNA replication process and could lead to a greater understanding of how some hereditary conditions develop.<\/p>\n<p>Jo Morris, Professor of Molecular Genetics in the Department of Cancer and Genomic Sciences at the University of Birmingham and corresponding author of the study said:<\/p>\n<p>\u201cOur study concerns how our cells use specific enzymes to support the typical regulation of DNA replication.<\/p>\n<p>\u201cWe found that because there are several different enzymes involved in cleaving and unwinding, cells must regulate which ones they use so that replication can happen properly. We identified that protein USP50 is involved in this regulation.<\/p>\n<p>\u201cThis discovery may be an important step to understanding how some hereditary gene changes lead to early onset ageing and cancer.\u201d<\/p>\n<h2 class=\"wp-block-heading\">Attempted workaround<\/h2>\n<p>The study also found that when USP50 is absent during replication activity, cells attempted to use various nucleases and helicases in a less coordinated fashion leading to replication defects in cells.<\/p>\n<p>Professor Morris added: \u201cThe finding that cellular nucleases and helicases can stop replication of certain sections of DNA was a surprise \u2013 it shows that cells closely co-ordinate their toolkit of DNA-processing enzymes to get DNA replication properly done.\u201d<\/p>\n<p>Professor Simon Reed, Co-Director of the Division of Cancer and Genetics at Cardiff University and a co-founder of\u00a0Broken String\u00a0Biosciences, and co-author of the paper said:<\/p>\n<p>\u201cI am truly honoured to have co-authored this paper published in\u00a0<em>Nature Communications<\/em>, exploring the critical role of\u00a0USP50\u00a0in safeguarding genome stability. This research sheds light on the complex mechanisms that protect our cells from DNA damage and highlights how these discoveries could shape future therapies.<\/p>\n<p>\u201cThank you to my collaborators\u2014together, we\u2019ve taken another step forward in understanding how our cells function and how we can apply this knowledge to advance medical science.\u201d<\/p>\n<h2 class=\"wp-block-heading\">About this genetics research news<\/h2>\n<p class=\"has-background\" style=\"background-color:#ffffe8\"><strong>Author: <\/strong><a href=\"http:\/\/neurosciencenews.com\/cdn-cgi\/l\/email-protection#74005a19150d1b34161c15195a15175a011f\" target=\"_blank\" rel=\"noreferrer noopener\">Tim Mayo<\/a><br \/><strong>Source: <\/strong><a href=\"https:\/\/bham.ac.uk\" target=\"_blank\" rel=\"noreferrer noopener\">University of Birmingham<\/a><br \/><strong>Contact: <\/strong>Tim Mayo \u2013 University of Birmingham<br \/><strong>Image: <\/strong>The image is credited to Neuroscience News<\/p>\n<p class=\"has-background\" style=\"background-color:#ffffe8\"><strong>Original Research: <\/strong>Open access.<br \/>\u201c<a href=\"https:\/\/www.nature.com\/articles\/s41467-024-52250-4\" target=\"_blank\" rel=\"noreferrer noopener\">USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication<\/a>\u201d by Simon Reed et al. <em>Nature Communications<\/em><\/p>\n<hr class=\"wp-block-separator has-text-color has-pale-cyan-blue-color has-alpha-channel-opacity has-pale-cyan-blue-background-color has-background\"\/>\n<p><strong>Abstract<\/strong><\/p>\n<p><strong>USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication<\/strong><\/p>\n<p>Mammalian DNA replication relies on various DNA helicase and nuclease activities to ensure accurate genetic duplication, but how different helicase and nuclease activities are properly directed remains unclear.<\/p>\n<p>Here, we identify the ubiquitin-specific protease, USP50, as a chromatin-associated protein required to promote ongoing replication, fork restart, telomere maintenance, cellular survival following hydroxyurea or pyridostatin treatment, and suppression of DNA breaks near GC-rich sequences.<\/p>\n<p>We find that USP50 supports proper WRN-FEN1 localisation at or near stalled replication forks.<\/p>\n<p>Nascent DNA in cells lacking USP50 shows increased association of the DNA2 nuclease and RECQL4 and RECQL5 helicases and replication defects in cells lacking USP50, or FEN1 are driven by these proteins.<\/p>\n<p>Consequently, suppression of DNA2 or RECQL4\/5 improves USP50-depleted cell resistance to agents inducing replicative stress and restores telomere stability.<\/p>\n<p>These data define an unexpected regulatory protein that promotes the balance of helicase and nuclease use at ongoing and stalled replication forks.<\/p>\n<p> <!-- Form created by Optin Forms plugin by WPKube: create beautiful optin forms with ease! --> <!-- https:\/\/wpkube.com\/ --><!--optinforms-form5-container--> <!-- \/ Optin Forms --> <\/div>\n<p><script async src=\"https:\/\/pagead2.googlesyndication.com\/pagead\/js\/adsbygoogle.js?client=ca-pub-3711241968723425\"\r\n     crossorigin=\"anonymous\"><\/script>\r\n<ins class=\"adsbygoogle\"\r\n     style=\"display:block\"\r\n     data-ad-format=\"fluid\"\r\n     data-ad-layout-key=\"-fb+5w+4e-db+86\"\r\n     data-ad-client=\"ca-pub-3711241968723425\"\r\n     data-ad-slot=\"7910942971\"><\/ins>\r\n<script>\r\n     (adsbygoogle = window.adsbygoogle || []).push({});\r\n<\/script><br \/>\n<br \/><div data-type=\"_mgwidget\" data-widget-id=\"1660802\">\r\n<\/div>\r\n<script>(function(w,q){w[q]=w[q]||[];w[q].push([\"_mgc.load\"])})(window,\"_mgq\");\r\n<\/script>\r\n<br \/>\n<br \/><a href=\"https:\/\/neurosciencenews.com\/dna-enzyme-genome-stability-27863\/\">Source link <\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Summary: Researchers have discovered that the protein USP50 regulates DNA replication by managing which enzymes\u2014nucleases or helicases\u2014cleave or unwind DNA strands during replication. This control is crucial for stable replication, &hellip; <a href=\"https:\/\/hotvideos24.online\/?p=114303\" class=\"more-link\">Read More<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8628],"tags":[],"class_list":["post-114303","post","type-post","status-publish","format-standard","hentry","category-science","entry"],"_links":{"self":[{"href":"https:\/\/hotvideos24.online\/index.php?rest_route=\/wp\/v2\/posts\/114303","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hotvideos24.online\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/hotvideos24.online\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/hotvideos24.online\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/hotvideos24.online\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=114303"}],"version-history":[{"count":0,"href":"https:\/\/hotvideos24.online\/index.php?rest_route=\/wp\/v2\/posts\/114303\/revisions"}],"wp:attachment":[{"href":"https:\/\/hotvideos24.online\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=114303"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hotvideos24.online\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=114303"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hotvideos24.online\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=114303"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}